Author(s):
Rajeh A, Alammouri Y, Alzehouri J
Email(s):
abeer.rajeh@damascusuniversity.edu.sy
DOI:
10.52711/0975-4385.2026.00033 
Address:
Rajeh A1, Alammouri Y2*, Alzehouri J3
1PhD Student, Department of Food and an Analytical Chemistry, Faculty of Pharmacy, Damascus University.
2Assistant Professor, International University for Science and Technology, Faculty of Pharmacy.
3Professor, Department of Food and an Analytical Chemistry, Faculty of Pharmacy, Damascus University.
*Corresponding Author
Published In:
Volume - 18,
Issue - 3,
Year - 2026
ABSTRACT:
Fenugreek (Trigonella foenum-graecum L.) is widely recognized for its nutritional and medicinal significance and is commonly used in traditional medicine as well as in functional food formulations. The whole plant, seeds, and sprouted seeds are known to contain a diverse range of bioactive compounds. The present study aimed to evaluate the biochemical composition of fenugreek callus tissue produced through in vitro plant tissue culture techniques. Callus tissue was induced from leaf explants of 15-day-old seedlings cultured on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), either alone or in combination with benzylaminopurine (BAP). Biochemical analyses were performed to determine mineral content, carbohydrate profile, and fatty acid composition. Mineral analysis revealed potassium as the predominant element in the callus tissue, along with detectable levels of sodium, calcium, iron, and zinc. Carbohydrate profiling using high-performance liquid chromatography (HPLC) confirmed the presence of soluble sugars, with fructose and raffinose identified as the dominant components. Lipid analysis conducted by gas chromatography–mass spectrometry (GC–MS) showed that the stored fats were mainly composed of unsaturated fatty acids, particularly a-linolenic acid, linoleic acid, and oleic acid. These findings support the suitability of MS medium supplemented with NAA, with or without BAP, for the cultivation of metabolically active fenugreek callus tissue. Overall, the results indicate that T. foenum-graecum callus tissue is biochemically diverse and represents a promising in vitro source of nutritionally and pharmaceutically valuable compounds.
Cite this article:
Rajeh A, Alammouri Y, Alzehouri J. Biochemical Characterization of Trigonella foenum-graecum Callus Tissue Cultivated in vitro. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(3):233-7. doi: 10.52711/0975-4385.2026.00033
Cite(Electronic):
Rajeh A, Alammouri Y, Alzehouri J. Biochemical Characterization of Trigonella foenum-graecum Callus Tissue Cultivated in vitro. Research Journal of Pharmacognosy and Phytochemistry. 2026; 18(3):233-7. doi: 10.52711/0975-4385.2026.00033 Available on: https://rjpponline.org/AbstractView.aspx?PID=2026-18-3-3
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