ABSTRACT:
Wild onions are an essential therapeutic herb. In several in vitro models, we studied Wild Onion extracts' phenolic components, antioxidant, anti-hyperglycemic, and anthelmintic properties. Wild onion pieces were extracted using several solvents in ascending order of polarity, including chloroform, ethyl acetate, methanol, ethanol, and distilled water. The antioxidant activity of wild onion extracts was assessed in a variety of in vitro model systems, including DPPH scavenging, ferric-reducing antioxidant power, and metal-chelating capacity. The phenolic content was also evaluated, and finally, an in vitro anthelmintic investigation was conducted out utilizing Phertima posthuma. In vitro studies revealed that several Wild Onion extracts have considerable DPPH Ferric-Reducing Antioxidant Power and Metal-Chelating Capacity scavenging activity. The findings revealed a clear link between phenolic acid concentration and antioxidant activity. The anthelmintic investigation found that the aqueous extract had the highest in-vitro activity, taking 3.52±0.158 min to paralyze and 2.74±0.247 min to kill earthworms. The experimental results gained in the laboratory model may give a foundation for the traditional usage of these pieces as antioxidants and anthelmintics. Our data show that the traditional medicinal claims for this plant, in the near future, will undoubtedly be able to replace the synthetic medications to which there is an increased incidence of drug interactions and resistance.
Cite this article:
Mayur Masidkar, Samruddhi Dange. Wild Onion Extraction and It’s Study on Antioxidant and Anthelmintic Property: An In-vitro Assessment. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(1):9-3. doi: 10.52711/0975-4385.2025.00003
Cite(Electronic):
Mayur Masidkar, Samruddhi Dange. Wild Onion Extraction and It’s Study on Antioxidant and Anthelmintic Property: An In-vitro Assessment. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(1):9-3. doi: 10.52711/0975-4385.2025.00003 Available on: https://rjpponline.org/AbstractView.aspx?PID=2025-17-1-3
REFERENCES:
1. Aggarwal H, Kotwal N. Foods used as ethno-medicine in Jammu. Studies on Ethno-Medicine. 2009 Jan 1;3(1):65-8.
2. Thippeswamy NB, Naidu KA. Antioxidant potency of cumin varieties—cumin, black cumin and bitter cumin—on antioxidant systems. European Food Research and Technology. 2005 May; 220:472-6.
3. Freeman GG, Mossadeghi N. Influence of sulphate nutrition on the flavour components of garlic (Allium sativum) and wild onion (A. vineale). Journal of the Science of Food and Agriculture. 1971 Jul; 22(7): 330-4.
4. Nguyen NH, Driscoll HE, Specht CD. A molecular phylogeny of the wild onions (Allium; Alliaceae) with a focus on the western North American center of diversity. Molecular Phylogenetics and Evolution. 2008 Jun 1; 47(3):1157-72.
5. Simin N, Mitić-Ćulafić D, Pavić A, Orcic D, Nemes I, Cetojevic-Simin D, Mimica-Dukić N. An overview of the biological activities of less known wild onions (genus Allium sect. Codonoprasum). Biologia Serbica. 2020 Feb 27;41(2).
6. Kousalya M, Geetha P, Jesuraja A, Kumar MV. In-Vitro study of anthelmintic activity of Eclipta prostrata (L) y various Extracts. Research Journal of Pharmacy and Technology. 2017; 10(1):58-60.
7. Babu ND, Kodithala S, Murali R, Srinivasan N. Anthelmintic activity of methanolic bark extract of Buchanania axillaris (Desr.). Research Journal of Pharmacy and Technology. 2018; 11(4): 1298-300.
8. Shagana S, Navenaa S, Vijay J, Vishali S, Rajendran V. Investigation of in vitro Anthelmintic activity of Ocimum basilicum Linn. (Lamiaceae). Research Journal of Pharmacy and Technology. 2021; 14(1):52-4.
9. Koleva II, Van Beek TA, Linssen JP, Groot AD, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques. 2002 Jan; 13(1):8-17.
10. Lim YY, Lim TT, Tee JJ. Antioxidant properties of several tropical fruits: A comparative study. Food chemistry. 2007 Jan 1;103(3):1003-8.
11. Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chemistry. 2007 Jan 1; 103(2):381-8.
12. Seifried HE, McDonald SS, Anderson DE, Greenwald P, Milner JA. The antioxidant conundrum in cancer. Cancer Research. 2003 Aug 1; 63(15): 4295-8.
13. Eswaraiah MC, Reddy DP, Illendula S. In-vitro anthelmintic activity of Tamilnadia uliginosa fruits. Research Journal of Pharmacy and Technology. 2019; 12(1): 321-3.
14. Sutar NG, Giri MA, Kendre PN, Syed NL, Singh J. Phytochemical Investigation and Pharmaological Screening of Alienthus excelsa Linn. Leaves as Anthelmentic activity in Earthworm. Research Journal of Pharmacy and Technology. 2010; 3(1): 244-6.
15. Tehseen F, Ghori SS, Dawood M, Tazeen S, Sara K, Samad A. Phytochemical screening and invitro anthelmintic activity of a novel polyherbal formulation. Research Journal of Pharmacy and Technology. 2021; 14(5): 2742-4.
16. Rajesh R, Selvakumar K. Evaluation of in vitro Anthelmintic activity of Lannea coromandelica (Houtt.) Merr. against Pheretima posthuma and Ascardia galli. Research Journal of Pharmacy and Technology. 2022; 15(6): 2539-42.
17. Ivanova AV, Gerasimova EL, Brainina KZ. Potentiometric study of antioxidant activity: development and prospects. Critical Reviews in Analytical Chemistry. 2015 Oct 2; 45(4):311-22.
18. Kang MY, Tsuchiya M, Packer L, Manabe M. In vitro study on antioxidant potential of various drugs used in the perioperative period. Acta Anaesthesiologica Scandinavica. 1998 Jan; 42(1):4-12.
19. Stephens NG, Parsons A, Brown MJ, Schofield PM, Kelly F, Cheeseman K, Mitchinson MJ. Randomised controlled trial of vitamin E in patients with coronary disease: Cambridge Heart Antioxidant Study (CHAOS). The Lancet. 1996 Mar 23; 347(9004): 781-6.
20. Mishra A, Pradhan DK, Mishra MR, Behera R, Panda AK, Vaishnaw SK. In-vitro Anthelmintic Activity of Musa sapientum Linn. Flower. Research Journal of Pharmacy and Technology. 2011; 4(1): 89-91.
21. Chiranjeevi U, Kamarajan K, Manna PK. Investigation of in-vitro anthelmintic activity of Lindernia madayiparense. Research Journal of Pharmacy and Technology. 2018; 11(1):183-6.
22. Gayathiri NM, Sudhakar P, Manimekalai P, Sabarinath C. In Vitro Anti-helminthic Activity of hydroalcoholic extract of Martynia annua L. and Pentanema indicum. Research Journal of Pharmacy and Technology. 2019; 12(8):3847-50.
23. Wu X, Gu L, Holden J, Haytowitz DB, Gebhardt SE, Beecher G, Prior RL. Development of a database for total antioxidant capacity in foods: a preliminary study. Journal of Food Composition and Analysis. 2004 Jun 1; 17(3-4):407-22.
24. MR A. Antioxidant study of pulps and peels of dragon fruits: a comparative study. International Food Research Journal. 2010; 17: 367-75.
25. Potapovich AI, Kostyuk VA. Comparative study of antioxidant properties and cytoprotective activity of flavonoids. Biochemistry (Moscow). 2003 May; 68:514-9.
26. Korotkova EI, Karbainov YA, Shevchuk AV. Study of antioxidant properties by voltammetry. Journal of Electroanalytical Chemistry. 2002 Jan 11; 518(1): 56-60.
27. Deepa VS, Kumar PS, Latha S, Selvamani P, Srinivasan S. Antioxidant studies on the ethanolic extract of Commiphora spp. African Journal of Biotechnology. 2009; 8(8).
28. Moon JK, Shibamoto T. Antioxidant assays for plant and food components. Journal of Agricultural and Food Chemistry. 2009 Mar 11; 57(5): 1655-66.
29. Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chemistry. 2007 Jan 1; 103(2):381-8.
30. Chaves N, Santiago A, Alías JC. Quantification of the antioxidant activity of plant extracts: Analysis of sensitivity and hierarchization based on the method used. Antioxidants. 2020 Jan 15; 9(1):76.
31. Veeru P, Kishor MP, Meenakshi M. Screening of medicinal plant extracts for antioxidant activity. Journal of Medicinal Plants Research. 2009 Aug 1; 3(8):608-12.