M.R. Agrawal, A.N. Aher, S.C. Pal
M.R. Agrawal1*, Dr. A.N. Aher2, Dr. S.C. Pal2
1Sitabai Thite College of Pharmacy, Shirur, Pune (District), Maharashtra-412210, India.
2MVP’s College of Pharmacy, Nashik, Maharashtra, India.
Volume - 12,
Issue - 3,
Year - 2020
The purpose of the study is to isolate and characterize the chemical constituents from fruits of Momordica balsamina. The principal theme of the study is to develop applied chromatographic techniques for the separation, isolation and detection of the compounds. The petroleum ether extract of fruits of Momordica balsamina was saponified and unsaponifiable matter was subjected to column chromatography andelution of column was carried out by Petroleum ether (100%) with increasing concentration of ethyl acetate for the separation of phytoconstituents. The isolated compounds were characterized and analyzed by physical characteristics, IR, NMR and Mass spectroscopy. Four known compounds lupeol, ursolic acid, ß-amyrin and ß-sitosterol were isolated from fruits of Momordica balsamina. The isolated compounds were quantitatively analyzed by HPTLC. The concentration of lupeol, ursolic acid and ß-amyrin was found to be 1.15%, 0.6% and 2.8%. From the present study, it is concluded that the plant consists of phytosterols and triterpene.
Cite this article:
M.R. Agrawal, A.N. Aher, S.C. Pal. Isolation, Characterization of Phytoconstituents from petroleum ether extract of Momordica balsamina fruits and its quantitative analysis by HPTLC. Res. J. Pharmacognosy and Phytochem. 2020; 12(3):156-161. doi: 10.5958/0975-4385.2020.00026.6
M.R. Agrawal, A.N. Aher, S.C. Pal. Isolation, Characterization of Phytoconstituents from petroleum ether extract of Momordica balsamina fruits and its quantitative analysis by HPTLC. Res. J. Pharmacognosy and Phytochem. 2020; 12(3):156-161. doi: 10.5958/0975-4385.2020.00026.6 Available on: https://rjpponline.org/AbstractView.aspx?PID=2020-12-3-6
1. Rasul MG, Hiramatsu M andOkubo H. Genetic relatedness (diversity) and cultivar identification by randomly amplified polymorphic DNA (RAPD) markers in Teasle gourd (Momordica dioica Roxb.). Scientia Horticulturae. 2007; 111: 271–279.
2. Kirtikar KR, Basu BD. Indian Medicinal Plants. International book publisher, India. 2005; 2: 1132-1137.
3. Bot YS, Mgbojikwe LO, Chika N, Alash’le A, Jelpe D and Demas D. Screening of the fruit pulp extract of Momordica balsamina for anti HIV property. African Journal of Biotechnology. 2007; 6 (1): 047-052.
4. Warrier PK, Nambiar VPK and Ramankutty C. Indian Medicinal Plant: A Compendium of 500 Species. Orient Longman. 1993; 4: 48.
5. Nadkarnis KM. Indian Material Medica. Popular publication, Mumbai, 2009. 2: 805.
6. Khandelwal KR. Practical Pharmacognosy. Nirali publication, Pune; 9: 139-168.
7. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy book, Nirali publication, Pune 2009; A.1.
8. Nariya PB, Shukla VJ, Acharya RN, Nariya MB, Bhatt PV, Pandit CM, Tada R. Isolation and characterization of phytosterols from Cordia macleodii (hook f. And thomson) bark by chromatographic and spectroscopic method. Asian J Pharm Clin Res 2014. 7(5): 86-88.
9. Fernandes P, CabralJMS. Phytosterols: Applications and recovery methods. Bioresource Technology 2007; 98: 2335–2350.
10. Wagner H and Bladt S. Plant drug analysis: A thin layer chromatography atlas. Springer: London. 1996; (2): 149-152 and 305-310.
11. Stahl E. Thin layer chromatography: A laboratory handbook. Springer: London. 2005; (2): 240-247.
12. Sutar RC, Kasture SB and Kalaichelvan VK. Fingerprinting analysis of the phytosterols from Holoptelea integrifolia (roxb.) planch leaves using high-performance thin layer chromatography analysis. Asian J Pharm Clin Res. 2014; 7(4): 160-164.
13. Mallick SS and Dighe VV. Detection and Estimation of alpha-Amyrin, beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography. Advances in Chemistry. 2014; 1-7.