Author(s):
Rajasekaran A, Mohanapriya B, Arivukkarasu R
Email(s):
arsekaran@gmail.com
DOI:
10.52711/0975-4385.2025.00042
Address:
Rajasekaran A*, Mohanapriya B, Arivukkarasu R
KMCH College of Pharmacy, Department of Pharmaceutical Analysis, Coimbatore, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 4,
Year - 2025
ABSTRACT:
The present study was aimed to scientifically validate the ayurvedic polyherbal tablet formulation used for the treatment of COVID-19. Organoleptic evaluation, physiochemical evaluation such as water-soluble extractive value, alcohol soluble extractive value, pH determination, weight variation, disintegration test, phytochemical investigation and HPTLC analysis, determination of microbial contamination and heavy metal analysis of the Ayurvedic Polyherbal Tablet Formulation were studied. The HPTLC analysis of Ayurvedic Polyherbal Tablet Formulation confirmed 0.04% of rutin in each methanol and aqueous extracts, 0.46% and 0.14% of quercetin, 0.04% and 0.17% of gallic acid, 0.19% of piperine in each methanol and aqueous extracts, 0.11 and 0.32% of andrographolide in methanol and aqueous extracts respectively. Except weight variation, remaining evaluation tests such as extractive value, disintegration time, pH and the phytochemical investigation of Ayurvedic Polyherbal Tablet Formulation were found to be within the pharmacopeial limits. HPTLC investigations of methanol and aqueous extracts of the Ayurvedic Polyherbal Tablet Formulation confirmed the presence of all ingredients mentioned in the Label claim. Microbial tests and heavy metal analysis revealed that the formulation is safe as it does not contain any microorganisms or heavy metals.
Cite this article:
Rajasekaran A, Mohanapriya B, Arivukkarasu R. HPTLC Standardization of an Ayurvedic Polyherbal Tablet for Covid-19 Management. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(4):258-8. doi: 10.52711/0975-4385.2025.00042
Cite(Electronic):
Rajasekaran A, Mohanapriya B, Arivukkarasu R. HPTLC Standardization of an Ayurvedic Polyherbal Tablet for Covid-19 Management. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(4):258-8. doi: 10.52711/0975-4385.2025.00042 Available on: https://rjpponline.org/AbstractView.aspx?PID=2025-17-4-2
REFERENCES:
1. Srikanth N, Ramesh Babu. Research and Development in Ayurveda –An overview. International Conference on Global scenario of Traditional system of Medicine, Ayurveda, Agriculture and Education. 2013.
2. Uma Narayanamurthy, Mirunalini R, Subha V, Manimekalai K, Sakthibalan K, Arther Paul C, Nagarajan KM and Sabarianandh JV. Acute and Reported Dose Toxicity Study of Clevira Syrup – A Polyherbal Formulation. Biomedical and Pharmacology Journal. 2021; 14(3):1459-1467.
3. Karthikeyan Lakshmanan, Arivukkarasu Ramasamy, Rajasekaran Aiyalu, Hari Baskar Balasubramanian, Anguraj Moulishankar. Elucidation of plausible mechanisms of kabasura kudineer for covid-19 by molecular docking against SARS COV-2. Indo American J of Pharmaceutical Sciences. 2020;7:((7):1795-1802.
4. Esther Lydia, Sheila John, Riyazudin Mohammed and Thiyagarajan Sivapriya. Investigation on the Phytochemicals present in the Fruit peel of Carica papaya and evaluation of its Antioxidant and Antimicrobial property. Research Journal of Pharmacognosy and Phytochemistry. 2016; 8(4):217-222.
5. Rajurkar RM, Jain RG, Bedmohta PA and Khadbadi SS. Antioxidant Activity of Phenolic Extract from Ginger (Zingiber officinale Roscoe) Rhizome. Asian Journal Research Chemistry. 2009;2(3):260-261
6. Lydia J. and Sudarsanam D. Total phenol and total tannin content of Cyperus rotundus L. and its medicinal significance. Research Journal of Pharmacy and Technology. 2012; 5(12):1500-1502.
7. Vijaya Kumar S, Dhirendra B, Sanghai Mallikarjuna Rao C, Shreedhara C. S. Antioxidant and antihyperlipidemic activity of Melia azedarach Linn. extracts in rats. Research Journal of Pharmacy and Technology. 2013; 6(11):1195-1199
8. Nirupama Rani Dewangan, Avantika Agrawal, Bharti Ahirwar. Novel Therapeutic Approach of Mollugo cerviana (L.) Ser Plant: A Review. Research Journal of Pharmacy and Technology. 2022; 15(7):3280-3284.
9. NB Ghiware and TM Nesari.Antipyretic Activity of Piper nigrum and Nyctanthes arbor-tristis in Different Dosage Forms. Research Journal of Pharmacy and Technology 2010;3(1): 157-160.
10. Ragavee A, Tenzin Choedar, Sourabh Suman, S. Asha Devi. Evaluation of Bioactive Constituents and Antioxidant Activity of Mucilage Isolated from Tinospora cordifolia. Research J. Pharm. and Tech 2018; 11(7): 2747-2751.
11. Wajid Azam, Vicky Rai, Rehan Jawed, Kalyani Jaywardhane, Vishal Thakur, Vishal Jain, Parwez Alam.Comparison of Antifungal and Antimicrobial activities in different parts of Carica papaya Linn. Asian Journal of Pharmaceutical Research. 2023;13(4):213-218.
12. Raj K. Tiwari R. Pandey S.S. Shukla, Prashant Tiwari, H. Shah. Antibacterial Activity of Aerial Part of Andrographis paniculata Research Journal of Pharmacognosy and Phytochemistry. 2014; 6(3): 122-125.
13. Lohar DR. Protocol for testing Ayurvedic, Siddha & Unani medicines, Department of AYUSH, Ministry of Health & Family Welfare, Pharmacopoeial Laboratory for Indian medicines. 2007.
14. Pulok K. Mukherjee. Quality control and evaluation of herbal drugs. Published by Elsevier Science. First Edition. 2019.
15. Chaowalit Monton, Worawan Saingam, Jirapornchai Suksaeree, Apirak Sakunpak, Krisana Kraisintu. Preformulation and Physical Properties study of Fast Disintegrating Tablets from Thai Traditional Formula. International Journal of Pharmacy and Pharmaceutical Sciences. 2014; 6(4): 431-434.
16. Pattanayak P, Mohapatra P, Jena RK and Panda SK. Standardization of Sulaharan Yoga: An Ayurvedic Tablet Formulation. Indian Journal of Pharmaceutical Sciences. 2011; 73(1): 65-70.
17. Quality control methods for medicinal plant materials. Published by WHO. Geneva. 1998;64-73.
18. Standard Operating Procedures. Determination of metals by Inductively Coupled Plasma methods. Published by Seras. 2006:1-42.
19. Marimuthu M, Mohanraj, Manojkumar D, Melina D. Cruz, Rajasekaran A, Arivukkarasu R. Estimation of Andrographolide Content in Aqueous and Methanolic Extracts of Marketed Nilavembu Kudineer Siddha formulations by HPTLC Technique. Asian Journal of Pharmaceutical Analysis. 2023:13(4): 239-242.
20. Rajasekaran A, Linda A, Arivukkarasu R. Estimation of Andrographolide Content in Aqueous Leaf Extract of Andrographis paniculata (Burm. f.) Wall. ex Nees from Different Regions of Tamilnadu and Kerala. Inventi Rapid: Pharm Analysis & Quality Assurance. 2016; 1-18.
21. Choudhary, Prassan, Chakdar, Hillol, Singh, Dikcha, Selvaraj, Chandrabose, Singh, Sanjeev Kumar, Kumar, Sunil, Saxena, Anil Kumar: Computational studies reveal piperine, the predominant oleoresin of black pepper (Piper nigrum) as a potential inhibitor of SARS-CoV-2 (COVID-19). Current Science. 2020; 119(8): 1333-1342.
22. Arivukkarasu R, Rajasekaran A, Sethuramani, Kattu raja, Mohamed Ali Jinna, Nandhini, Ramya M Vinothini R. Detection and estimation of well-known free radical scavengers rutin, quercetin and gallic acid in market herbal anti-inflammatory and anti- arthritis formulations by HPTLC Methods. Scholars Academic Journal of Pharmacy (SAJP). 2017; 6(5): 221-228.
23. Nourah Mohammad Abd El-Aziz, Mohamed G. Shehata, Olfat M. Eldin Awad, Sobhy Ahmad El-Sohaimy. Inhibition of COVID-19 RNA Dependent RNA Polymersae by Natural Bioactive Compounds: Molecular Docking Analysis. Egyptian Journal of Chemistry. 2021; 64(4): 1989-2001.
24. Pawan K. Agrawal, Chandan Agarwal, Gerald Blunden. Rutin: A potential antiviral for repurposing as a SARS-CoV-2 Main Protease inhibitor. Natural Product Communications. 2021; 1694: 1-12.
25. Ali Saeedi-Boroujeni, Mohammad Reza, Mahmoudian Sani. Anti-inflammatory potential of Quercetin in COVID-19 treatment. Journal of Inflammation. 2021; 18(3): 1-11.