ABSTRACT:
Shoots were regenerated from the cultured roots of Oldenlandia umbellata L. successfully for mass propagation on Murashige and Skoog (MS) medium. The nodal shoots were cultured on 3.0 mgl-1 Benzylaminopurine (BAP) to induce nodal meristems. The shoots were multiplied well on MS liquid medium supplemented with 1.0 mgl-1 BAP + 0.5 mgl-1 Indole-3 acetic acid (IAA). Shoots were rooted on half strength MS medium supplemented with 2.5 mgl-1 Indole-3 butyric acid (IBA). The in vitro produced roots were further used as explants to initiate root cultures to get secondary metabolites (anthraquinone derivatives/dye). The root pieces were cultured on half strength MS medium supplemented with IBA. The multiplied roots turned yellow in color within 4-5 weeks. Shoots were regenerated from the roots when the roots cultured on MS medium augmented with 1.0 mgl-1 BAP. Again these shoots were rooted with help of IBA and the plantlets were hardened in green house.