Author(s): Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani

Email(s): uswah_apt@ub.ac.id

DOI: 10.52711/0975-4385.2022.00027   

Address: Uswatun Khasanah1*, Oktavia Rahayu Adiningsih1, Ernila Dewi Anggraeni2, Firda Uswatul Uliyah2, Annisa Intan Ramadhani2, Aloysia Aprilla Dewi Saraswati2, Gita Kurnia Ardiani2
1Department of Pharmacy, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
2Undergraduate Student of Pharmacy Study Program, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2022


ABSTRACT:
The exploration of plants as a source of medicinal raw materials has increased since the last few decades, both for infectious, degenerative, and malignant diseases. One of the medicinal plants currently being developed for cancer is Syzygium cumini. Powder of Syzygium cumini was extracted using 96% ethanol. Each extract was determined for its phytochemical screening, total phenolic content, and cytotoxic activity against 4T1 cell culture. Total phenolic content was determined by the Folin-Ciocalteu method and UV-VIS spectrophotometer. Cytotoxic test was performed using MTT assay with parameter IC50 value. The results showed that the total phenolic content of 96% ethanol extract of Syzygium cumini seed, leaf, and pulp was 503.01±9.21; 329.60± 20.37; 24.09 ±0.57mg GAE/g. Syzygium cumini seed, leaf, and pulp extracts exhibited cytotoxic activity with IC50 613.92±40.49; 660.18±15.02; 732.68±69.41µg/ml. Syzygium cumini seed extract has the highest total phenolic content and the most potent cytotoxic activity.


Cite this article:
Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani. Phytochemical screening, Total Phenolic content and Cytotoxic Activity of Seed, Leaves, and Pulp from Syzygium cumini against Breast Cancer Cell Culture 4T1. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(3):145-9. doi: 10.52711/0975-4385.2022.00027

Cite(Electronic):
Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani. Phytochemical screening, Total Phenolic content and Cytotoxic Activity of Seed, Leaves, and Pulp from Syzygium cumini against Breast Cancer Cell Culture 4T1. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(3):145-9. doi: 10.52711/0975-4385.2022.00027   Available on: https://rjpponline.org/AbstractView.aspx?PID=2022-14-3-1


REFERENCES:
1.    Angahar LT. An Overview of Breast Cancer Epidemiology, Risk Factors, Pathophysiology, and Cancer Risks Reduction. MOJ Biol Med. 2017;1(4):92–6.
2.    American Cancer Society. Breast Cancer Risk and Prevention Breast Cancer Risk Factors You Cannot Change. CancerOrg. 2020;1–45.
3.    Masuda H, Zhang D, Bartholomeusz C, Doihara H, Hortobagyi GN, Ueno NT. Role of epidermal growth factor receptor in breast cancer. Breast Cancer Res Treat. 2012;136(2):331–45.
4.    Hernawati S. The inhibition of malignant epithelial cells in mucosal injury in the oral cavity of strains by pomegranate fruit extract (Punica granatum linn) through Bcl-2 expression. Dent J (Majalah Kedokt Gigi). 2013;46(1):35.
5.    DiPiro JT, Yee GC, Posy M, Haines ST, Nolin TD, Ellingrod V. Pharmacotherapy: A Pathophysologic Approach, 11th Edition. 11th ed. New York: McGraw Hill; 2020.
6.    Alimuddin Saifi, Rajani Chauhan, Jaya Dwivedi. Assessment of the antidiabetic activity of Syzygium cumini (Linn.) Skeels in alloxan induced diabetic rats. Res. J. Pharmacology & Pharmacodynamics.2016; 8(3): 91-96. doi: 10.5958/2321-5836.2016.00017.3
7.    Sathish Babu. P, Gokula Krishnan, Anand Babu.K, Chitra. K. In silico and In vitro Evaluation of Anti-urolithiatic Activity of Ethanolic Extract of Syzygium cumini Stem Bark. Research J. Pharm. and Tech. 2017; 10(5): 1317-1321. doi: 10.5958/0974-360X.2017.00233.5.
8.    Amutha K, Aishwarya S. Evaluation of Antibacterial and Antidiabetic Activity and Phytochemical Analysis of Syzygium cumini (l.) Skeels. Seed. Research J. Pharmacology and Pharmacodynamics. 2010; 2(5): 348-350.
9.    Nikhat F., Satyanarayana D., Suresh D.K., Purohit M.G., Raza H. , Hamza S.M. Analgesic Activity of the Isolated Constituent Syzygium cuminii (L) skeel. Research J. Pharmacognosy and Phytochemistry. 2011; 3(4): 178-179.
10.    Yadav SS Meshram GA, Shinde D, Patil RC, Manohar SM, Upadhye M V. Antibacterial and Anticancer Activity of Bioactive Fraction of Syzygium cumini L. Seeds. HAYATI J Biosci [Internet]. 2011;18(3):118–22. Available from: http://dx.doi.org/10.4308/hjb.18.3.118
11.    Tripathy G. In-Vitro Anti Breast Cancer Activity of Syzygium Cumini Against MCF-7 Cell Line. J Innov Pharm Biol Sci. 2015;2(2):119–24.
12.    Shrikanta A, Kumar A, Govindaswamy V. Resveratrol content and antioxidant properties of underutilized fruits. J Food Sci Technol. 2015;52(1):383–90.
13.    Fu Y, Chang H, Peng X, Bai Q, Yi L, Zhou Y, et al. Resveratrol inhibits breast cancer stem-like cells and induces autophagy via suppressing Wnt/β-catenin signaling pathway. PLoS One. 2014;9(7).
14.    Imene Guediri, Chérifa Boubekri, Ouanissa Smara, Touhami Lanez. Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (black nightshade) growing in the south of Algeria. Asian J. Research Chem. 2021; 14(1):47-55. doi: 10.5958/0974-4150.2021.00008.0
15.    Anupam Kumar, Anu, Anand Mohan, Neeta Raj Sharma, Hasibur Rehman. Antibacterial, Antioxidant analysis of Phytochemical Extracts derived from seeds of Syzygium cumini L. against Pathogenic Bacteria. Research J. Pharm. and Tech. 2017; 10(8): 2707-2712. doi: 10.5958/0974-360X.2017.00481.4
16.    Kothari V, Seshadri S, Mehta P. Fractionation of antibacterial extracts of Syzygium cumini (Myrtaceae) seeds. Res Biotechnol [Internet]. 2011;2(6):53–63.
17.    D. Sheela, Maria Cheenickal. Total Phenolics and Flavonoids among The Selected Species of Syzygium, Gaertn. Res. J. Pharmacognosy and Phytochem. 2017; 9(2): 101-104. doi: 10.5958/0975-4385.2017.00018.8
18.    Neelam Jain, Shaily Goyal, K. G. Ramawat. Radical Scavenging Activity and Total Phenolic Content in Selected Fruits and Vegetables. Research J. Pharm. and Tech. 2012; 5(1): 121-124.
19.    Eun SY, Ko YS, Park SW, Chang KC, Kim HJ. P2Y2 nucleotide receptor-mediated extracellular signal-regulated kinases and protein kinase C activation induces the invasion of highly metastatic breast cancer cells. Oncol Rep. 2015;34(1):195–202.
20.    Kampa M, Alexaki VI, Notas G, Nifli AP, Nistikaki A, Hatzoglou A, et al. Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action. Breast Cancer Res. 2004;6(2):63–74.
21.    Ávila-Gálvez MÁ, Giménez-Bastida JA, Espín JC, González-Sarrías A. Dietary phenolics against breast cancer. A critical evidence-based review and future perspectives. Int J Mol Sci. 2020;21(16):1–33.
22.    Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula S V. An overview on the role of dietary phenolics for the treatment of cancers. Nutr J [Internet]. 2016;15(1):1–16. Available from: http://dx.doi.org/10.1186/s12937-016-0217-2
23.    Menaka M, Chandra Venkatasubramanian. Free radical scavenging activity of Lyophilised Syzygium cumini (L) Skeels fruit pulp (Jamun). Research J. Pharm. and Tech. 2017; 10(4): 986-990. doi: 10.5958/0974-360X.2017.00179.2
24.    Singletary KW, Jung KJ, Giusti M. Anthocyanin-rich grape extract blocks breast cell DNA damage. J Med Food. 2007;10(2):244–51.
25.    Tan J, Li Q, Xue H, Tang J. Ultrasound-assisted enzymatic extraction of anthocyanins from grape skins: optimization, identification, and antitumor activity. J Food Sci. 2020;85(11):3731–44.
26.    Lin BW, Gong CC, Song HF, Cui YY. Effects of anthocyanins on the prevention and treatment of cancer. Br J Pharmacol. 2017;174(11):1226–43.

Recomonded Articles:

Author(s): Mr. Avinash B. Thalkari, Mr. Pawan N. Karwa, Ms. Pallavi S. Shinde, Chandrakant S. Gawli, Priyanka S. Chopane

DOI: 10.5958/0975-4385.2020.00006.0         Access: Open Access Read More

Author(s): Gaurav Kumar, Indu Sharma

DOI:         Access: Open Access Read More

Author(s): Shantha Sheela Nagarajan, Muthusamy Periyannan , Radha Ramalingam

DOI: 10.5958/0975-4385.2016.00013.3         Access: Open Access Read More

Author(s): K Amutha , D Victor Arokia Doss

DOI:         Access: Open Access Read More

Author(s): Gottumukkala Krishna Mohan, Malavika Yadav, M Sandhya Rani, Kalakotla Shanker

DOI: 10.5958/0975-4385.2016.00033.9         Access: Open Access Read More

Author(s): Manoj Tripathi, Alok Kumar, Anil Kumar, Virendra Rajak

DOI: 10.5958/0975-4385.2017.00027.9         Access: Open Access Read More

Author(s): Swathi Sree Karumuri, Venkata Naveen Kasagana.

DOI:         Access: Open Access Read More

Author(s): Vikas Gupta, Junaid Niazi, Jatinderjot Kaur Kehal, Parveen Bansal

DOI:         Access: Open Access Read More

Author(s): Dhanya Rajan, J. Suresh, N. Paramakrishnan, A. Sri Vasavi Reddy, M Nayeem

DOI:         Access: Open Access Read More

Author(s): Syed Safiullah Ghori, Mohib Khan, Mohammed Shamim Qureshi

DOI: 10.5958/0975-4385.2015.00003.5         Access: Open Access Read More

Author(s): Ravi Sunder Prajapati, G.P. Richhariya, I. P. Tripathi, Ravindra Singh, Manoj Tripathi

DOI: 10.5958/0975-4385.2016.00012.1         Access: Open Access Read More

Author(s): Veeresh P Veerapur, Pramod P Desai, S Vijayakumar

DOI: 10.5958/0975-4385.2018.00046.8         Access: Open Access Read More

Author(s): Anjali Soni, Patel Femida, Preeti Sharma

DOI: 10.5958/0975-4385.2017.00003.6         Access: Open Access Read More

Author(s): Priya Dongare, Swati Dhande, Vilasrao Kadam

DOI:         Access: Open Access Read More

Author(s): D. Esther Lydia, Sheila John, Swetha V K, Thiyagarajan Sivapriya

DOI: 10.5958/0975-4385.2017.00045.0         Access: Open Access Read More

Author(s): Puri Abhijeet V, Ansari Yunus N, Puri Priti A

DOI: 10.5958/0975-4385.2017.00026.7         Access: Open Access Read More

Research Journal of Pharmacognosy and Phytochemistry (RJPP) is an international, peer-reviewed journal, devoted to pharmacognosy...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4385 


Recent Articles




Tags