Author(s):
Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani
Email(s):
uswah_apt@ub.ac.id
DOI:
10.52711/0975-4385.2022.00027 
Address:
Uswatun Khasanah1*, Oktavia Rahayu Adiningsih1, Ernila Dewi Anggraeni2, Firda Uswatul Uliyah2, Annisa Intan Ramadhani2, Aloysia Aprilla Dewi Saraswati2, Gita Kurnia Ardiani2
1Department of Pharmacy, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia.
2Undergraduate Student of Pharmacy Study Program, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
*Corresponding Author
Published In:
Volume - 14,
Issue - 3,
Year - 2022
ABSTRACT:
The exploration of plants as a source of medicinal raw materials has increased since the last few decades, both for infectious, degenerative, and malignant diseases. One of the medicinal plants currently being developed for cancer is Syzygium cumini. Powder of Syzygium cumini was extracted using 96% ethanol. Each extract was determined for its phytochemical screening, total phenolic content, and cytotoxic activity against 4T1 cell culture. Total phenolic content was determined by the Folin-Ciocalteu method and UV-VIS spectrophotometer. Cytotoxic test was performed using MTT assay with parameter IC50 value. The results showed that the total phenolic content of 96% ethanol extract of Syzygium cumini seed, leaf, and pulp was 503.01±9.21; 329.60± 20.37; 24.09 ±0.57mg GAE/g. Syzygium cumini seed, leaf, and pulp extracts exhibited cytotoxic activity with IC50 613.92±40.49; 660.18±15.02; 732.68±69.41µg/ml. Syzygium cumini seed extract has the highest total phenolic content and the most potent cytotoxic activity.
Cite this article:
Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani. Phytochemical screening, Total Phenolic content and Cytotoxic Activity of Seed, Leaves, and Pulp from Syzygium cumini against Breast Cancer Cell Culture 4T1. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(3):145-9. doi: 10.52711/0975-4385.2022.00027
Cite(Electronic):
Uswatun Khasanah, Oktavia Rahayu Adiningsih, Ernila Dewi Anggraeni, Firda Uswatul Uliyah, Annisa Intan Ramadhani, Aloysia Aprilla Dewi Saraswati, Gita Kurnia Ardiani. Phytochemical screening, Total Phenolic content and Cytotoxic Activity of Seed, Leaves, and Pulp from Syzygium cumini against Breast Cancer Cell Culture 4T1. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(3):145-9. doi: 10.52711/0975-4385.2022.00027 Available on: https://rjpponline.org/AbstractView.aspx?PID=2022-14-3-1
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