Author(s): Sourav Chakraborty, Sumedha Saha, Malay Bhattacharya

Email(s): malaytsnbu@gmail.com

DOI: 10.52711/0975-4385.2023.00044   

Address: Sourav Chakraborty1,2, Sumedha Saha1, Malay Bhattacharya1*
1Molecular Biology and Tissue Culture Laboratory, Department of Tea Science, University of North Bengal, Siliguri, Darjeeling, West Bengal, India, 734013.
2Post Graduate Department of Botany, Darjeeling Government College, Darjeeling, West Bengal, India, 734101.
*Corresponding Author

Published In:   Volume - 15,      Issue - 4,     Year - 2023


ABSTRACT:
Silver nanoparticles (AgNP’s) are 1 - 100nm sized constituents providing additional surface area in formation of plentiful coordinate bonds with various ligands. Green mode of AgNP’s synthesis has aided in development of advanced formulations towards treating complex human ailments. Herpetospermum darjeelingense (C.B.Clarke) H. Schaef. and S.S. Renner is an under investigated endemic ethnomedicinally significant cucurbit climber with no work based on synthesis and exploration of biogenic AgNP’s being conducted previously employing its solvent extracts. This paper aims to provide insights on the green synthesized AgNP’s besides studying its antimicrobial, antioxidant and hepatoprotective efficacy. Methanolic extracts of H. darjeelingense were utilized to synthesize AgNP’s via reduction of silver nitrate following UV-Visible spectroscopic and scanning electron microscopic characterization depicting surface plasmon resonance bands at around 430nm wavelength with spherical to cubical shaped AgNP’s ranging between 40–80nm. The methanol based sample extracts did not portray any inhibition zone in antimicrobial assay with the AgNP’s showing considerable zone of inhibition against Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus indicating anti-microbial effectiveness of silver nano formulations. The highest free radical scavenging potential was shown by 50mg/ml (91.99%) silver nano formulation with minimal being recorded in 12.5mg/ml (50.11%) nanoparticle concentrate including a noted gradual increase in anti-lipid peroxidation ability from 12.5mg/ml (7.157±0.08273mg/ml Tocopherol equivalent) to 50mg/ml (18.128±0.03152mg/ml Tocopherol equivalent) nanoparticle formulation clearly portraying statistically significant elevated results in comparison to plain sample extracts depicting its anticipated usage in pharmaceutical applications concerning antibacterial, antioxidative and hepatoprotective features.


Cite this article:
Sourav Chakraborty, Sumedha Saha, Malay Bhattacharya. Synthesized green silver nano particles of Herpetospermum darjeelingense shows enhanced In vitro Antimicrobial, Antioxidant and Hepatoprotective activity. Research Journal of Pharmacognosy and Phytochemistry. 2023; 15(4):281-7. doi: 10.52711/0975-4385.2023.00044

Cite(Electronic):
Sourav Chakraborty, Sumedha Saha, Malay Bhattacharya. Synthesized green silver nano particles of Herpetospermum darjeelingense shows enhanced In vitro Antimicrobial, Antioxidant and Hepatoprotective activity. Research Journal of Pharmacognosy and Phytochemistry. 2023; 15(4):281-7. doi: 10.52711/0975-4385.2023.00044   Available on: https://rjpponline.org/AbstractView.aspx?PID=2023-15-4-3


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