Author(s):
Devendra Surendra Mahale, Tejas Gautam Jain, Rajesh A. Ahirrao, Hemant P. Suryawanshi
Email(s):
devendramahale2306@gmail.com
DOI:
10.52711/0975-4385.2025.00027
Address:
Devendra Surendra Mahale*, Tejas Gautam Jain, Rajesh A. Ahirrao, Hemant P. Suryawanshi
Shri. Govardhansingh ji Shaikshanik Seva Samittee’s P. G. College of Pharmaceutical Science and Research,
At Post- Chaupale, Tal. and Dist. Nandurbar 425411 (MS).
*Corresponding Author
Published In:
Volume - 17,
Issue - 2,
Year - 2025
ABSTRACT:
In a number of sectors, such as pharmaceuticals, food production, and environmental control, enzyme biotechnology is essential. Enzyme immobilization, which involves binding enzymes to solid supports, improves their stability, reusability, and efficiency. This review focuses on this procedure. The article examines the various techniques for immobilizing enzymes, including adsorption, covalent bonding, entrapment, and encapsulation. Each technique has special benefits, including improved process control, decreased contamination, and better stability. In pharmaceutical applications, immobilized enzymes are frequently utilized for high-purity active pharmaceutical ingredients (APIs), drug manufacturing, and enzyme-based drug delivery. The article also emphasizes their function in vaccine manufacture and biosensors for disease diagnostics. It also looks at how immobilized plant and bacterial cells can be used for environmental applications such as bioremediation, biotransformation, and the synthesis of secondary metabolites. Enzyme immobilization's overall importance stems from its capacity to maximize industrial processes, making them more economical, efficient, and sustainable. Enzymes continue to provide creative answers to a variety of biotechnological problems through a variety of immobilization approaches.
Cite this article:
Devendra Surendra Mahale, Tejas Gautam Jain, Rajesh A. Ahirrao, Hemant P. Suryawanshi. A Comprehensive Review on Enzyme Immobilization Techniques and their Applications in Biotechnology. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(2):168-2. doi: 10.52711/0975-4385.2025.00027
Cite(Electronic):
Devendra Surendra Mahale, Tejas Gautam Jain, Rajesh A. Ahirrao, Hemant P. Suryawanshi. A Comprehensive Review on Enzyme Immobilization Techniques and their Applications in Biotechnology. Research Journal of Pharmacognosy and Phytochemistry. 2025; 17(2):168-2. doi: 10.52711/0975-4385.2025.00027 Available on: https://rjpponline.org/AbstractView.aspx?PID=2025-17-2-14
REFERENCE:
1. Dhanasekaran, D., and Thavapalan, M. Enzyme Biotechnology and Its Applications in Industry. Journal of Industrial Microbiology and Biotechnology. 2019; 46(2): 285-294. https://doi.org/10.1007/s10295-019-02242-5
2. Singh, R. S., and Yadav, R. Applications of Enzyme Technology in Biotechnology and Industry. International Journal of Engineering Research and Technology. 2017; 6(1): 45-55. Retrieved from: https://www.ijert.org/research/applications-of-enzyme-technology-in-biotechnology-and-industry-IJERTV6IS010229
3. Mateo, C., Palomo, J. M., Fernandez-Lorente, G., and et al. Immobilization of Enzymes. Biochemical Engineering Journal. 2007; 37(2): 95-107. https://doi.org/10.1016/j.bej.2007.01.021
4. Gupta, M. N., and Gupta, S. (2015). Enzyme Immobilization: Applications and Prospects. Comprehensive Biotechnology (2nd ed., Vol. 3). Elsevier. ISBN: 978-0-08-087779-9
5. Fernández-Lafuente, R. Immobilized Enzymes: Carriers, Support Materials and Applications. Methods in Enzymology. 2010; 470: 31-50. https://doi.org/10.1016/S0076-6879(10)70003-0
6. Wang, C., and Sunde, J. Plant Cell Immobilization for Pharmaceutical Production. Biotechnology Advances. 2012; 30(4):735-741. https://doi.org/10.1016/j.biotechadv.2012.02.004
7. Gerhart, M., and Ayala, M. Applications of Immobilized Enzymes in Pharmaceutical Drug Manufacturing. Biotechnology for Pharmaceutical Applications. 2018; 30(2): 101-108. https://doi.org/10.1002/btpr.2382