Author(s): Khan Wasim Raza Ali, Chaurey Mayur, Gupta Mansi


DOI: 10.52711/0975-4385.2023.00031   

Address: Khan Wasim Raza Ali1*, Chaurey Mayur2, Gupta Mansi2
1School of Pharmacy, ITM (SLS) Baroda University, Halol - Vadodara Highway, Vadodara, 391510 Gujarat, India.
2Corporate Institute of Pharmacy, Anand Nagar, Bhopal, 462021, MP, India.
*Corresponding Author

Published In:   Volume - 15,      Issue - 3,     Year - 2023

Ficus hispida Linn. is a small but well-distributed species of the tropical fig tree. Although used in traditional medicine to treat many ailments, there is a lack of system reports about the phytochemical and antioxidant properties of the leaves of that plant. This study was conducted to investigate the phytochemical and antioxidant properties of F. hispida leaves using four different solvents (methanol, ethanol, chloroform, and petroleum ether). Qualitative and quantitative analysis are performed to determine the presence and content of phytochemicals. Four in vitro assay models were used to investigate the antioxidant activity. Phytochemical experiments confirmed the presence of alkaloids, flavonoids, flavonols, resins, saponins, proanthocyanidins, glycosides, steroids, and tannins. Qualitative analysis showed the total content of polyphenols such as phenolic, flavonoids, flavonols, and proanthocyanidin in those extracts. Among all the Extracts, ethanol extracted from the leaves of F. hispida has shown superior activity in the testing of the total antioxidant capacity assay. In DPPH and ABTS scavenging, ethanol extracted from F. hispida leaves shows the highest scavenging activity among all extractions with IC50 values of 50.33±2.59µg/ml and 83.35±1.98µg/ml respectively. Although in Superoxide radical scavenging assay and Nitric Oxide radical scavenging assay, methanol extraction of F. hispida leaves exhibit with an IC50 value of 104.17±3.49µg/ml and 108.98±4.66µg/ml.

Cite this article:
Khan Wasim Raza Ali, Chaurey Mayur, Gupta Mansi. A Study on Phytochemical and Antioxidant properties of the Leaves of Ficus hispida Linn. Research Journal of Pharmacognosy and Phytochemistry. 2023; 15(3):203-8. doi: 10.52711/0975-4385.2023.00031

Khan Wasim Raza Ali, Chaurey Mayur, Gupta Mansi. A Study on Phytochemical and Antioxidant properties of the Leaves of Ficus hispida Linn. Research Journal of Pharmacognosy and Phytochemistry. 2023; 15(3):203-8. doi: 10.52711/0975-4385.2023.00031   Available on:

1.    Rakhi Menon. Antioxidants and their Therapeutic Potential- A Review. Research J. Pharm. and Tech. 6(12): Dec. 2013; 1426-1429.
2.    Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing, 2011; 89: 217-233.
3.    R Jasmine, Manikandan K, Karthikeyan K. Evaluating the antioxidant and anticancer property of Ficus carica fruits. African Journal of Biotechnology, 2015; 14(7): 634-641.
4.    Selvakumar K., Madhan R., Srinivasan G., Baskar V. Antioxidant Assays in Pharmacological Research. Asian J. Pharm. Tech. 1(4): Oct. - Dec. 2011; 99-103.
5.    Kumaraswamy MV, Satish S. Antioxidant and Anti Lipoxygenase activity of Thespesia lampas Dalz & Gibs, Advan. Biol. Res., 2008; 2(3-4): 56-59.
6.    Jadhav Sameer S., Salunkhe Vijay R. Magdum Chandrakant S. Daily Consumption of Antioxidants:-Prevention of Disease is better than Cure. Asian J. Pharm. Res. 3(1): Jan.-Mar. 2013; 33-39.
7.    Maharaja P, Sengottuvel T, Aarthi A, Gopalasatheeskumar K. Review on Antioxidant and Hepatoprotective activity of Medicinal plants against Paracetamol Induced animal model. Res. J. Pharmacognosy and Phytochem. 2020; 12(2): 114-119. doi: 10.5958/0975-4385.2020.00020.5
8.    O.M. Ighodaro and O.A. Akinloye. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid./Alexandria Journal of Medicine, 2018; 54: 287–293.
9.    Kris Etherton PM, Hecker KD, Bonanome A, Cival SM, Binkasi AE, Hilpert KF. Bioactive compounds in foods, their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine, 2002; 113: 71-88.
10.    Grice HP. Enhanced tumor development by butylated hydroxyanisole (BHA) from the perspective of effect on forestomach and oesophageal squamous epithelium. Food Chem. Toxicol, 1988; 26: 717-723.
11.    Witschi HP. Safety evaluation of butylated hydroxytoluene (BHT) in the liver, lung, and gastrointestinal tract. Food Chem Toxicol, 1986; 24: 1127-1130.
12.    Li L, Ham H, Sung J, Kim Y, Lee HJJ. Antioxidant activities of methanolic extracts from four different rose cultivars. Journal of Food and Nutrition Research, 2014; 2(2): 69-73.
13.    Baker JC, Owens RA, Whitaker BD, Mock NM, Roberts DP, Deahl KL, et al. Effect of viroid infection on the dynamics of phenolic metabolites in the apoplast of tomato leaves. Physiol Mol Plant Pat., 2008; 74(3-4): 214-220.
14.    Sivaraman D, Muralidharan P. Anti-ulcerogenic evaluation of root extract of ficus hispida linn: In aspirin ulcerated rats. Afr J Pharma Pharmacol. 2010; 4: 72–82.
15.    The useful plants of India. Available:
16.    Ali M and Chaudhary N. Ficus hispida Linn.: A review of its pharmacognostic and ethnomedicinal properties. Pharmacogn Rev., 2011; 5(9): 96–102
17.    Salvi V, Joshi Y, Dhande S, Kadam V. A review on Ficus hispida. Research Journal of Pharmacognosy and Phytochemistry, 2013; 5(3): 149-154.
18.    Sivaraman D, Muralidaran P. Acetylcholine enhancing the activity of methanol leaf extract of Ficus hispida Linn in rat hippocampus. J Herbal Med Toxicol, 2009; 3: 147–50.
19.    Mandal SC, Kumar CK. Studies on the anti-diarrhoeal activity of Ficus hispida leaf extract in rats. Fitoterapia, 2002; 73: 663-7.
20.    Mandal SC, Saraswathi B, Kumar CK, Lakshmi SM, Maiti BC. Protective effect of leaf extract of Ficus hispida Linn. Against paracetamol-induced hepatotoxicity in rats. Phytothet Res, 2000; 14: 457- 9
21.    Pratumvinit B, Srisapoomi T, Worawattananon P, Opartkiattikul N, Jiratchariyakul W, et al. In vitro antineoplastic effect of Ficus hispida L. Plant against breast cancer cell lines. J Med Plants Res, 2009; 3: 255–61.
22.    Pandit K, Karmarkar SM, Bhagwat AM. Evaluation of anti-hyperlipidemic activity of Ficus hispida Linn leaves in Triton WR-1339 (Tyloxopol) induced hyperlipidemia in mice. Int J Pharm Pharm Sci., 2011; 3(Suppl 5): 188-191.
23.    Hossain S, Parvin S, Dutta S, Islam K, Saha MR, et al. Evaluation of antimicrobial activities of methanolic extract and fractions of Ficus hispida Linn. Fruits. Int. J. Pharm., 2014; 5(5): 390-393.
24.    Murti K, Lambole V, Panchal M. Effect of Ficus hispida on normal dexamethasone suppressed wound healing. Braz. J. Pharm. Sci., 2011; 47(4): 855-860.
25.    Sivaraman D, Muralidharan P. Anti-ulcerogenic evaluation of root extract of Ficus hispida Linn: In aspirin ulcerated rats. Afr J Pharma Pharmacol, 2010; 4: 72-82.
26.    Johansen DA. Plant micro technique, New York, 1940: 182.
27.    Chandrashekar K, Santanu S, Prasanna K. Phytochemical studies of aerial parts of the plant Leucas lavandulaefolia. Der Pharma Chemica, 2010; 2(5): 434-437.
28.    Abu Bakar MF, Mohamed M, Rahmat A, Fry J. Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chemistry, 2009; 113: 479–483.
29.    Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extracts of Phyllanthus species from India. LWT- Food Sci Technol, 2007; 40: 344– 352.
30.    Sun JS, Tsuang YW, Chen IJ, Huang WC, Hang YS, Lu FJ. An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury. Burns, 1998; 24: 225–231.
31.    Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Bio., 1999; 269: 337–341.
32.    Shirwaikar A, Shirwaikar A, Rajendran K, Punitha IS. In vitro antioxidant studies on the benzyl tetra isoquinoline alkaloid berberin. Biol Pharm Bull, 2006; 29: 1906-1910.
33.    Kumar RS, Rajkapoor B, Perumal P. Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. Using various in vitro assay models. Asian Pac J Trop Biomed, 2012; 2(4): 256-261.
34.    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 1999; 26: 1231-1237.
35.    Sabu MC, Ramadasan K. Antidiabetic activity of medicinal plants and its relationship with their antioxidant property. J ethnopharmacol, 2002; 81: 155-160.
36.    Zhang T, Wei X, Miao Z, Hassan H, Song Y, Fan M. Screening for antioxidant and antibacterial activities of phenolics from Golden Delicious apple pomace. Chemistry Central Journal, 2016; 10: 47.
37.    Lou SN, Lai YC, Hsu YS, Ho CT. Phenolic content, antioxidant activity and effective compounds of kumquat extracted by different solvents. Food Chem, 2016; 197: 1-6.
38.    Sankhalkar S, Vernekar V. Quantitative and Qualitative Analysis of Phenolic and Flavonoid Content in Moringa oleifera Lam and Ocimum tenuiflorum L. Pharmacogn Res, 2016; 8: 16-21. doi:10.4103/0974-8490.171095.
39.    Scalbert A, Manach C, Morand C, Remesy C: Dietary Polyphenols and the Prevention of Diseases. Cri Rev Food Sci Nut., 2005; 45: 287–306.
40.    Ariga T. The antioxidative function, preventive action on disease and utilization of proanthocyanidins. Bio Fact, 2004; 21: 197–2019.
41.    Rajurkar NS, Gaikwad K. Evaluation of phytochemicals, antioxidant activity and elemental content of Adiantum capillus veneris leaves. J. Chem. Pharm. Res., 2012; 4(1): 365-374.
42.    Pisoschi AM, Negulescu GP. Methods for total antioxidant activity determination:A review. Biochem & Anal Biochem, 2011; 1: 106.
43.    Kumarappan CT, Thilagam E, Mandal SC. Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens. Saudi J Biol Sci, 2012; 19: 349-355.

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