Henna is most commonly used for coloring the hair from more than 5000 years. It contains Lawsone which interacts with keratin present in skin and hair by Michael addition which results into permanent stain. Chemically Lawsone is 2-hydroxy-1,4-naphthoquinone found in the leaves of the henna plant as well as in the flower of water hyacinth. But the Lawsone isolation from Lawsonia inermis can be difficult due to its easily biodegradable nature. Hence isolation of Lawsone is done by using Column chromatography. The isolated extract was also tested for antimicrobial activity keeping Vancomycin as a standard. It has shown prominent Antimicrobial activity.
Cite this article:
Utkarsha Shivsharan, Yash Kothari. Antimicrobial Activity and Isolation of Lawsone from Lawsonia inermis using Column Chromatography. Res. J. Pharmacognosy and Phytochem. 2020; 12(4):219-223. doi: 10.5958/0975-4385.2020.00036.9
Utkarsha Shivsharan, Yash Kothari. Antimicrobial Activity and Isolation of Lawsone from Lawsonia inermis using Column Chromatography. Res. J. Pharmacognosy and Phytochem. 2020; 12(4):219-223. doi: 10.5958/0975-4385.2020.00036.9 Available on: https://rjpponline.org/AbstractView.aspx?PID=2020-12-4-5
1. CC Jones; The Encyclopaedia of Henna; US Library; USA; 2000; 952-968
2. ME Kraeling, RL Bronaugh, CT Jung; Absorption of lawsone through human skin; 2007;26(1):45-56
3. B HüseyinBoz; p-Coumaric acid in cereals: presence, antioxidant and antimicrobial effects; Volume 50, Issue 11, pages 2323–2328,
4. AKhatkar, A Nanda, Pkumar, Balasubramaniam, Arabian Journal of Chemistry, 2014.05.018
5. SWatak, SS. Patil, Asian J. Pharm. Ana. 2012; Vol. 2: Issue 2, Pg 52-67
6. A.A Warra, J. Chem. Pharm. Res., 2011, 3(4):951-958
7. YU Gadkari and VVelingkar, Journal of Chemical and Pharmaceutical Research, 2014, 6(11):14-19
8. A Mishra and V. S. Velingkar, Journal of Chemical and Pharmaceutical Research, 2015, 7(1):541-545
9. M. TerezaFernandeza, M. Lurdes Miraa b., M. Helena Flor'encioa, Keith R. Jennings, Journal of Inorganic Biochemistry (2002) 92,105-111.
10. Omael, Organo-metallic Intermolecular-coordination compounds, Elsevier Science Publication Amsterdam-VI, (1986).1-25
11. EG Rochow, organ metallic chemistry, Chapman and Hall Ltd., London, (1965).1-18 and 44-45
12. G.E Ryschkewitcher., Chemical Bonding and the Geometry of the molecules Reinhold Publications, New York, (1963). 92-95
13. K.D Tripathi., Essentials of Medical Pharmacology, Jaypee Brothers Medical Publishers, New Delhi, (2002),4th edition, 671-886
14. J.G Hardman, L.E Limbird et al, Goodman and Gillman's The Pharmacological Basis of Therapeutics, 9th edition McGraw-Hill Health Professions Division, New York, (1996), 1029-1046,
15. Marjorie Murphy Cowan Clinical Microbiology Reviews (1999), Vol-12,564-582
16. W. C. Evans, “Trease and Evans Pharmacognosy” Nirali Prakashan, (2008), 42nd edition, 205-223,
17. R.B Sykes, C.M Clamanistic. Et al nature, (1981).291,489-491
18. Astbury W. T. Weibull C. Nature, (1949), 163, 281-282
19. Aggarwal, B Bharat, Sundaram, Chitra; Malani, Nikita; Ichikawa, Haruyo (2007).
20. B Narasimhan, D Belsare, D Pharande, V Morya, A Dhake, European Journal of Medicinal Chemistry;(2004) 39 827-834.
21. The Merck Index: An Encyclopedia of chemicals, Drugs, and Biologicals (12thed.).
22. H. Wayne Richardson “Copper Compounds” in Ullmann's Encyclopedia of Industrial Chemistry 2005, Wiley-VCH, Weinheim.
23. R. B. Thurman, C. P. Gerba CRC Critical reviews in Environmental Control;(1989). 18 (4): 295-315.
24. Domek, MJ Robbins, JE Anderson, ME McFeters, Canadian Journal of Microbiology (1987). 57-62