Author(s):
Bharadhan Bose, Sethuramani Amarnathan, Gopi Selvam
Email(s):
b.barani143@gmail.com
DOI:
10.52711/0975-4385.2024.00016
Address:
Bharadhan Bose1,2*, Sethuramani Amarnathan2, Gopi Selvam2
1Sree Abirami College of Pharmacy, (Affiliated with The Tamil Nadu Dr. M. G. R. Medical University, Chennai) Coimbatore - 641021, Tamil Nadu, India.
2College of Pharmacy, Madurai Medical College, (Affiliated with The Tamil Nadu Dr. M. G. R. Medical University, Chennai) Madurai - 625020, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 2,
Year - 2024
ABSTRACT:
Objectives: Morinda coreia has rich active components which has been used in folk medicine for the treatment of several diseases. Their active substance content may vary according to plant genetics, parts used,as well as to climatic factors, quality of the ground in which the plants were grown, the time of harvesting, and the extraction methods. Basically the flowers can heal the common cold, improve memory, aid relaxation due to presence of volatile oil. Flowers could be used as a new approach for the development of nutraceutical products or functional foods. The flower part of this plant are not documented. To develop and apply it, we report Pharmacological effects such as anti-inflammatory, antimicrobial activity. Methods: The extracts were prepared by cold maceration process with aqueous and organic solvents such as ethanol and ethyl acetate. The anti-inflammatory activity were evaluated using carrageenan-induced paw edema method and the antimicrobial activity by agar well diffusion method. Results: The maximum zone of inhibition for Bacillus subtilis was found to be more susceptible toward the ethanolic extract (14mm) at the maximum conc. of 300µl followed by ethyl acetate (10mm) and aqueous extract (12mm). Similarly for Klebsiella pneumonia maximum inhibitory zone (13mm) followed Pseudomonas auroginosa (13mm), Staphylococcus aureus (12mm), Candida albicans was moderate sensitive towards ethanolic extract (11mm) Conclusion: The ethanolic extract of Morinda coreia flower possessed high anti-inflammatory, antimicrobial activity than ethyl acetate and aqueous extracts on comparing with standards.
Cite this article:
Bharadhan Bose, Sethuramani Amarnathan, Gopi Selvam. Screening of Anti-inflammatory and Antimicrobial activity on Flower Extracts of Morinda coreia Buch Ham. Research Journal of Pharmacognosy and Phytochemistry. 2024; 16(2):83-8. doi: 10.52711/0975-4385.2024.00016
Cite(Electronic):
Bharadhan Bose, Sethuramani Amarnathan, Gopi Selvam. Screening of Anti-inflammatory and Antimicrobial activity on Flower Extracts of Morinda coreia Buch Ham. Research Journal of Pharmacognosy and Phytochemistry. 2024; 16(2):83-8. doi: 10.52711/0975-4385.2024.00016 Available on: https://rjpponline.org/AbstractView.aspx?PID=2024-16-2-5
REFERENCES:
1. Diet, Nutrition and the Prevention of Chronic Diseases. World Health Organization, Technical Report Series, WHO, Geneva. 2003; 916.
2. Maver T, Maver U, StanaKleinschek K, Smrke DM, Kreft S. A review of herbal medicines in wound healing. Int J Dermatol. 2015; 54(7):740–751. doi: 10.1111/ijd.12766.
3. Elzayat EM, Auda SH, Alanazi FK, Al-Agamy MH. Evaluation of wound healing activity of henna, pomegranate and myrrh herbal ointment blend. Saudi Pharm J. 2018
4. Dons T, Soosairaj S. Evaluation of wound healing effect of herbal lotion in albino rats and its antibacterial activities. Clin Phytosci. 2018; 4(1): 6. doi: 10.1186/s40816-018-0065-z.
5. Pereira LOM, Vilegas W, Tangerina MMP, Arunachalam K, Balogun SO, Orlandi-Mattos PE, de Oliveira Martins DT. Lafoensiapacari A. St.-Hil.: wound healing activity and mechanism of action of standardized hydroethanolic leaves extract. J Ethnopharmacol. 2018; 219:337–350. doi: 10.1016/j.jep.2018.02.038.
6. Dorjsembe B, Lee HJ, Kim M, Dulamjav B, Jigjid T, Nho CW. Achilleaasiatica extract and its active compounds induce cutaneous wound healing. J Ethnopharmacol. 2017; 206:306–314. doi: 10.1016/j.jep.2017.06.006.
7. Singh H, Ali SS, Khan NA, Mishra A, Mishra AK. Wound healing potential of Cleome viscosa Linn. Seeds extract and isolation of active constituent. S Afr J Bot. 2017; 112: 460–465. doi: 10.1016/j.sajb.2017.06.026.
8. Muthu C, Ayyanar M, Raja N, Ignacimuthu S. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. J EthnobiolEthnomed. 2006; 2(1):43. doi: 10.1186/1746-4269-2-43.
9. Kirtikar KRBB, Basu BD. Indian medicinal plants. Indian Medicinal Plants. 2. Dehra Dun: K.A. Longman; 1935. pp. 1294–1295.
10. Jayasinghe ULB, Jayasooriya CP, Bandara BMR, Ekanayake SP, Merlini L, Assante G. Antimicrobial activity of some Sri Lankan Rubiaceae and Meliaceae. Fitoterapia. 2002; 73(5):424–427. doi: 10.1016/S0367-326X(02)00122-3
11. Kumaresan PT, Saravanan A. Anticonvulsant activity of Morinda tinctoria Roxb. Afr J Pharm Pharmacol. 2009;3(2):063–065. [Google Scholar]
12. Deepti K, Umadevi P, Vijayalakshmi G. Antimicrobial activity and phytochemical analysis of Morinda tinctoria Roxb leaf extracts. Asian Pac J Trop Biomed. 2012; 2(3):S1440–S1442. doi: 10.1016/S2221-1691(12)60433-X. [CrossRef] [Google Scholar]
13. Subramanian M, Balakrishnan S, Chinnaiyan SK, Sekar VK, Chandu AN. Hepatoprotective effect of leaves of Morinda tinctoria Roxb. againstparacetamol induced liver damage in rats. Drug Invent Today. 2013; 5(3):223–228. doi: 10.1016/j.dit.2013.06.008.
14. Sreena KP, Poongothai A, Soundariya SV, Srirekha G, Santhi R, Annapoorani S. Evaluation of in vitro free radical scavenging efficacy of different organic extracts of Morinda tinctoria leaves. Int J Pharm Pharm Sci. 2011; 3(Suppl 3):207–209.
15. Amerasan D, Murugan K, Panneerselvam C, Kanagaraju N, Kovendan K, Kumar PM. Bioefficacy of Morinda tinctoria and Pongamiaglabra plant extracts against the malaria vector Anopheles stephensi (Diptera: Culicidae) J EntomolAcarol Res. 2015;47(1):31–40. doi: 10.4081/jear.2015.1986.
16. Sivakumar T, Sivamaruthi BS, Priya KL, Kesika P, Chaiyasut C. Evaluation of bioactivities of Morinda tinctoria leaves extract for pharmacological applications. Evaluation. 2018; 11(2):100–105.
17. P. Thirupathy Kumaresan and A. Saravanan. Anticonvulsant activity of Morinda tinctoria-Roxb. African Jr of Pharmacy and Pharmacology. 2009; 3(2):063-065.
18. Subramaniyan Vijayakumar., Srinivasan Prabhu, and Sellen Chandrasekhar. Anti-Inflammatory activity of Morinda tinctoria on Carrageenan induced Rat Paw Edema. Int J Med Biosci. 2012; 1(4): 55 – 60.
19. Anand M, Muralidharan P. Antioxidant activity of Methanolic fruit extract of Morinda tinctoriaRoxb in Cerebral Ischemia Induced by Bilateral Common Carotid artery occlusion in rats. American Journal of Pharmtech Research 2014; 4(2):1-14.
20. Isha I. Sait, Jyoti Harindran, A. Abdul Vahab, Jeena J. L, Nasli S. Jolly John. Potential hepatoprotective effect and antioxidant role of methanol extract of Morinda tinctoria in carbon tetrachloride induced hepatotoxicity in albino rats. Int J Pharm. 2014; 4(1): 363-368.
21. Kanchanapoom T. Iridoid and phenolic glycosides from Morinda coreia. Phytochemistry. 2001; 59(5); 551-556. doi: 10.1016/s0031-9422(01)00426-5
22. Menone ML, Pesce SF, Diaz MP, Moreno VJ, Wunderlin DA. Endosulfan induces oxidative stress and changes on detoxification enzymes in the aquatic macrophyte Myriophyllumquitense. Phytochemistry. 2008; 69:1150-1157.
23. Ghasanfari G, Minaie B, Yasa N, Leilu AN, Azadeh M. Biochemical and histopathological evidences for beneficial effects of SaturejaKhuzestanicaJamzad essential oil on the mouse model of inflammatory bowel diseases. Toxicol Mech. 2006; 16:365-372
24. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of aging. Nature. 2000; 408:239-247.
25. Hartwell JL. Plants used against cancer. Quarterman: Lawrence, MA; 1982.
26. Mariappan V, Shanthi G, Saridha D. Pharmacognostical Studies on Morinda tinctoria. Roxb. Int J Pharm Pharm Sci. 2012; 4(2): 636-638.
27. Atish K Sahoo, Nisha Narayanan, N Satheesh Kumar, S Rajan, and Pulok K Mukherjee. Phytochemical and therapeutic potentials of Morinda tinctoriaRoxb. (Indian mulberry). Oriental Pharmacy and Experimental Medicine. 2009; 9(2): 101-105.
28. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of Cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinigenesis. 1989; 10: 1003-8.
29. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phoshomolybdenum complex: Specific application to the determination of vita min E. Analytical Biochemistry. 1999; 269:337–341.
30. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and Cytotoxicity assays. Journal of Immunological Methods. 1983; 65: 55-63.
31. Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P., Vaigro-Wolff, A., Gray-Goodrich, M., Campbell, H., Mayo, J., Boyd, 1991. Feasibility of high flux anticancer drug screen using a diverse panel of cultured human tumour cell lines. Journal of the National Cancer Institute. 83; 757-766.
32. Pooja A.S Bawa and Farhath Khanum. Anti-inflammatory activity of Rhodiolarosea- a second generation adaptogen. Phytother. Res. 2009; 23: 1099-1102.
33. Laouer H, Meriem, EK, Parado S, Baldovini N. An antibacterial and antifungal phenylpropanoid from C. monataum. Phytother Res. 2009; 23: 1726-30.