Formulation and Evaluation of Fenugreek powder as Nutraceutical from seeds of Trigonella foenum graecum


Chitra Govind Rajput

Ashokarao Mane College of Pharmacy, Peth, Vadgaon.

*Corresponding Author E-mail:



Fenugreek seeds are rich source of protein, minerals, vitamins, gum, fiber, alkaloid, flavonoids, saponin and volatile compounds Fenugreek (Trigonella foenum-graecum) is a legume and it has been used as a spice throughout the world to enhance the sensory quality of foods. It is known for its medicinal qualities such as antidiabetic, anticarcinogenic, hypocholesterolemic, antioxidant, and immunological activities. There for in this study we prepared the fenugreek powder as nutraceutical from seeds of Trigonella foenum graecum. It is evaluated for chemical test, particle size.


KEYWORDS: Nutraceutical, Fenugreek seed.




Nutraceutical is defined as- “a food (or a portion of food) that essentially provides distinct health and medical benefits, even including the prevention and/or treatment of particular disease.” The word “nutraceutical” is a portmanteau of the words “nutrition” and “pharmaceutical”, coined in 1989 by Stephen L. Defelice. Simply, Nutraceuticals means, Nutritive + Pharmaceutical: A food stuff (as a fortified food or dietary supplement) that provides health benefits.1


Fenugreek (Trigonella foenum-graecum) is a legume and it has been used as a spice throughout the world to enhance the sensory quality of foods. It is known for its medicinal qualities such as antidiabetic, anticarcinogenic, hypocholesterolemic, antioxidant, and immunological activities. Beside its medicinal value, it is also used as a part of various food product developments as food stabilizer2 It belongs to the legume plants and is an environmentally friendly plant.


The seeds are rich in biogenic element such as phosphorus, sulfur, magnesium, calcium but were less abundant in zinc, manganese and copper. Fenugreek seeds and leaves contain biologically active substances (protein, amino acids, biogenic elements, lipids and fatty acids)3


Fenugreek (Trigonella foenum graecum), native to southern Europe and Asia, is an annual herb with white flowers and hard, yellowish brown and angular seeds, known from ancient times, for nutritional value beside of it medicinal effects. Fenugreek seeds are rich source of gum, fiber, alkaloid, flavonoids, saponin and volatile content. Due to it's high content of fiber,4. Fenugreek (Trigonella foenum-graecum L) commonly known as methi is an annual herb belonging to the family Fabaceae. Fenugreek seeds are rich source of protein, minerals, vitamins, gum, fiber, alkaloid, flavonoids, saponin and volatile compounds. It is one of the most promising medicinal herbs, known from ancient times and shows antioxidant, anticarcinogenic, antidiabetic, hypocholesteromic, hypoglycemic and lactation induced properties.


Nutritional Value of Fenugreek:

Nutritional Facts Quantity/100g: Calories 323kcal, Total fat 6.41g, Dietary fiber 24.6g. Total Carbohydrate 58.35 g, Protein 23g.


Vitamins Quantity/100g: Vitamin A 3mg., Vitamin B1 (Thiamine) 0.322mg, Vitamin B2 (Riboflavin) 0.366mg, Vitamin B3 (Niacin) 1.64mg, Vitamin B-6 0.6mg, Vitamin B9 57µg, Vitamin C 3mg.


Minerals Quantity/100g: Calcium 176mg, Iron 33.53 mg, Magnesium 191mg, Sodium 67mg, Potassium 770 mg, Zinc 2.5mg. Manganese 1.23mg, Phosphorus 296 mg, Other constituents: Water – 9%




Plant materials was obtained from a Ayurvedic Medical Shop in Peth Vadgaon. Seeds are identified and authenticated by Department of Botany, Shivaji University, Kolhapur.

Chemical reagents:

Molisch’s Reagent (Sulphuric acid + alpha napthol), Biuret Reagent (Copper sulfate + base), Ethanol, Water.




1.     Required quantity of fenugreek seeds are weighed and taken.

2.     Seeds are then roasted in a frying pan until it becomes golden brown to minimize it’s bitterness.

3.     Then the roasted fenugreek seeds are powdered by suitable method.

4.     Powdered fenugreek seeds are sieved to get uniform and desired particle size.

5.     Other ingredients such as liquorice powder, mannitol, cocoa powder is added in suitable amount.

6.     And the powder is then stored in suitable air tight container to avoid moisture.



Chemical Test:

Sr. No.





Test for carbohydrate

Molisch’s Test - Few drops of solution of crude drug sample + Molisch’s Reagent (Sulphuric acid+alpha-napthol)

Purple ring at junction of two solutions

Carbohydrate                     present


Test for Protein

Biuret Test – Few drops of solution of crude drug sample + Biuret Reagent (Copper sulfate

+ Base)

Pink or Purple

Protein                              present


Test for Fat

Emulsion Test – Sample solution + Ethanol + Water

Solution becomes turbid

Fat                                    present



Sl. No.

Name of Ingredients




Fenugreek powder

100 g

Nutritional use



20 g



Liquorice powder

5 g

Natural Sweetener/Expectorant


Cocoa powder

10 g

Flavoring Agent




Fenugreek Power Supplement


Mfg. date- June 2020

Mfg. Lic. No.- 5457

Batch No.C5457

Exp. Date- Best before 6 months after manuhactyring.



Fenugreek Powder


Liquorice powder




Cocoa powder


Srorage – Store is well closed air tight container to avoid moisture

Use- Nutritional supplement

Direction- 1Scoop (10g) of powder mix water or milk and drink it daily

Manufactured by- Ashokrao mane college of Pharmacy, Peth Vadgaon



Evaluation Parameters:

Particle size:

Particle size is by far the most important physical property of powder material. A particle size determination is routinely carried out across a wide range of industries and is often critical parameter in the manufacture of many pharmaceutical powder formulations. A wide range of methods are available to determine the particle size.


The methods used to determine the particle size are:


Sedimentation Rate


Sieving method-

By using sieves of different mesh size the particle size of the powder can be determined as per IP.

In the sieving method different sieves with different mesh size is used and the powder is passed through it. The sieves are arranged in vertical with decrease in mesh size. The powder material is passed through it by agitating the sieve by hand or by mechanical shaker for a sufficient period of time. The powder material is collected from each sieve separately and the average weight is determined



Sl. No.

Grade of powder

Sieve no. through which all particles must pass





Moderately coarse



Moderately fine






Very fine


Powder have fine particle size



The powder of Fenugreek seeds from Trigonella foenum-graecum was prepared successfully, and evaluated for chemical test and particle size



Nutraceuticals have proven health benefits and their consumption will keep disease at bay and allow humans to maintain an overall good health. The powder formulation of fenugreek seeds are having a good nutritional value and health benefits and it provide easy way to get rid of some nutritionally induced acute and chronic diseases. As rich source of proteins, lipids, fatty acids and minerals the fenugreek seeds cater to the body’s needs for essential nutrients and deliver numerous health benefits.


Fenugreek can be recommended and must be taken as a part of our daily diet as its liberal use is safe and various health benefits can be drawn from this natural herb.



1.      "Trigonella foenum-graecum”. Germplasm Resources Information Network (GRIN). Agricultural Research Service (ARS), United States Department of Agriculture (USDA). Retrieved 2008-03-13.

2.      Shashikumar JN, Champawat PS, Mudgal VD and Jain SK, Role of fenugreek (Trigonella foenum graecum) on in management of diabetes disease, Journal of Pharmacognosy and Phytochemistry 2019; 8(4): 184-187

3.      Żuk-Gołaszewska K., Wierzbowska J. Fenugreek: productivity, nutritional value and uses., J. Elem., 2017, 22(3): 1067-1080

4.      Nasim Khorshidian, Amir Mortazavian, Abolfazl Adeli Mirzaie, Fenugreek: Potential Applications as a Functional Food and Nutraceutical, Vol no 1Jan -March 2016 Page 1-16

5.      Tabasum Fatima, Khushnuma Maqbool and Syed Zameer Hussain; Potential health benefits of fenugreek; Journal of Medicinal Plants Studies 2018; 6(2): 166-169






Received on 08.01.2021         Modified on 26.01.2021

Accepted on 05.02.2021  ©AandV Publications All right reserved

Res. J. Pharmacognosy and Phytochem. 2021; 13(2):78-80.

DOI: 10.52711/0975-4385.2021.00013