Author(s): Resa P. Parmar, Niranjan S. Kanaki

Email(s): resapparmar@gmail.com

DOI: 10.5958/0975-4385.2018.00024.9   

Address: Resa P. Parmar1*, Niranjan S. Kanaki2
1Lecturer, B. K. Modi Government Pharmacy College, Rajkot.
2Associate Professor, K. B. Institute of Pharmaceutical Education and Research, Gandhinagar.
*Corresponding Author

Published In:   Volume - 10,      Issue - 2,     Year - 2018


ABSTRACT:
Background: Boswellic acid, curcumin and diosgenin are important phytoconstituents present in Boswellia serrata, Curcuma longa and Trigonella foenum-graecum, respectively. Traditionally, these plants are used in the treatment of arthritis. In the present study an attempt has been made to develop a simple, precise, rapid, selective and cost-effective high-performance thin-layer chromatographic (HPTLC) method for simultaneous estimation of boswellic acid, curcumin and diosgenin from polyherbal transdermal gel prepared for the treatment of arthritis. Material and method: The method employed TLC aluminium plates precoated with silica gel 60F254 as the stationary phase. The solvent system consisted of hexane and acetone. Densitometric analysis was carried out in the absorbance mode at 423nm for curcumin whereas for boswellic acid and diosgenin, plates were scanned at 540nm after derivatization. Result: This system was found to give compact spots for boswellic acid (Rf value of 0.54), curcumin (Rf value of 0.30), and diosgenin (Rf value of 0.64). Response was a linear function of the amount applied to the plate in the ranges 1-6 µg for boswellic acid, curcumin and diosgenin. The % of boswellic acid, curcumin and diosgenin from transdermal gel was found to be 99.46%, 99.47% and 98.85% respectively, which was well within the limit. Conclusion: The developed HPTLC method would be an important tool in the quality control method for polyherbal formulations.


Cite this article:
Resa P. Parmar, Niranjan S. Kanaki. Development of Simultaneous HPTLC Method for estimation of Boswellic acid, Curcumin and Diosgenin in Polyherbal Transdermal Gel. Res. J. Pharmacognosy and Phytochem. 2018; 10(2): 163-167. doi: 10.5958/0975-4385.2018.00024.9


Recomonded Articles:

Author(s): Punita R. Maurya, Swati R. Dhande, Yadunath M. Joshi, Vilasrao J. Kadam

DOI:         Access: Open Access Read More

Author(s): Chitra Shenoy, M B Patil, Ravi Kumar

DOI:         Access: Open Access Read More

Author(s): Shantha Sheela Nagarajan, Muthusamy Periyannan , Radha Ramalingam

DOI: 10.5958/0975-4385.2016.00013.3         Access: Open Access Read More

Author(s): Shruti Srivastava, Jatin Jaiswal, Hemendra Gautam, Sunil Singh, Surabhi Sharma

DOI:         Access: Open Access Read More

Author(s): Pramod S. Shinde, Vashali J. Mahadik, Sushil M. Sarvagod

DOI: 10.5958/0975-4385.2016.00018.2         Access: Open Access Read More

Author(s): Soosamma John, Madhavi T., Bincy Raj, Jincy Shaji, Vinutha

DOI:         Access: Open Access Read More

Author(s): Kalpana Patil, Swati Dhande, Vilasrao Kadam

DOI:         Access: Open Access Read More

Author(s): Ravindra Singh, Aakanksha Tiwari

DOI: 10.5958/0975-4385.2017.00009.7         Access: Open Access Read More

Author(s): S. C. Verma, R. Rani, E. Vashishth, K. Basu, P. Pant, M. M. Padhi

DOI:         Access: Open Access Read More

Author(s): Mamta Tiwari, Pushpraj S. Gupta, Nisha Sharma

DOI: 10.5958/0975-4385.2018.00014.6         Access: Open Access Read More

Author(s): Dhanya Rajan, J. Suresh, N. Paramakrishnan, A. Sri Vasavi Reddy, M Nayeem

DOI:         Access: Open Access Read More

Author(s): Manoj Tripathi, Alok Kumar, Anil Kumar, Virendra Rajak

DOI: 10.5958/0975-4385.2017.00027.9         Access: Open Access Read More

Author(s): Bairagi V.A., Shinde P.R., Senthikumar K.L., Sandu N.

DOI:         Access: Open Access Read More

Author(s): Ravi Sunder Prajapati, G.P. Richhariya, I. P. Tripathi, Ravindra Singh, Manoj Tripathi

DOI: 10.5958/0975-4385.2016.00012.1         Access: Open Access Read More

Author(s): Raje VN, Yadav AV, Shelar PA

DOI:         Access: Open Access Read More

Author(s): Kirteebala P Pawar, Milind J Bhitre, Priyanka V Kalamkar, Mohan K Kale

DOI: 10.5958/0975-4385.2015.00013.8         Access: Open Access Read More

Author(s): Prabhudutta Panda, Maitrayee Panda, Siva Shankar Nayak , Durga Prasad Panda

DOI:         Access: Open Access Read More

Research Journal of Pharmacognosy and Phytochemistry (RJPP) is an international, peer-reviewed journal, devoted to pharmacognosy...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4385 


Recent Articles




Tags