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Volume No. :   5

Issue No. :  1

Year :  2013

ISSN Print :  0975-2331

ISSN Online :  0975-4385


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Induction of shoots from in vitro cultured roots of Oldenlandia umbellata L. – a dye-yielding plant



Address:   M.S. Shekhawat*, N. Kannan, M. Manokari, J. Revathi
K.M. Centre for Postgraduate Studies, Pondicherry-605 008, India *Corresponding Author
DOI No:

ABSTRACT:
Shoots were regenerated from the cultured roots of Oldenlandia umbellata L. successfully for mass propagation on Murashige and Skoog (MS) medium. The nodal shoots were cultured on 3.0 mgl-1 Benzylaminopurine (BAP) to induce nodal meristems. The shoots were multiplied well on MS liquid medium supplemented with 1.0 mgl-1 BAP + 0.5 mgl-1 Indole-3 acetic acid (IAA). Shoots were rooted on half strength MS medium supplemented with 2.5 mgl-1 Indole-3 butyric acid (IBA). The in vitro produced roots were further used as explants to initiate root cultures to get secondary metabolites (anthraquinone derivatives/dye). The root pieces were cultured on half strength MS medium supplemented with IBA. The multiplied roots turned yellow in color within 4-5 weeks. Shoots were regenerated from the roots when the roots cultured on MS medium augmented with 1.0 mgl-1 BAP. Again these shoots were rooted with help of IBA and the plantlets were hardened in green house.
KEYWORDS:
Anthraquinone derivatives, Oldenlandia umbellata L., root culture, shoot regeneration
Cite:
M.S. Shekhawat, N. Kannan, M. Manokari, J. Revathi. Induction of shoots from in vitro cultured roots of Oldenlandia umbellata L. – a dye-yielding plant. Research Journal of Pharmacognosy and Phytochemistry. 2013; 5(1): 22-25.
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