Preeti Tiwari, Rakesh K.Patel
Preeti Tiwari1* and Rakesh K.Patel2
1Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001,Gujarat, India
2Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711,Gujarat, India
Volume - 4,
Issue - 5,
Year - 2012
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Draksharishta-T and Draksharishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin–Ciocalteu reagent and was found 0.0967 and 0.0961 %w/w gallic acid equivalent in Draksharishta-T and Draksharishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01163 and 0.01129 %w/w quercetin equivalent in Draksharishta-T and Draksharishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Draksharishta-T and Draksharishta-M. The antioxidant activity of Draksharishta-T and Draksharishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Draksharishta-T and Draksharishta-M showed significant scavenging of super-oxide radical and showed IC50 138.06 and 145.35 µg/ml respectively. Draksharishta-T and Draksharishta-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 230.03 and 236.11 µg/ml respectively. Marketed Draksharishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Draksharishta-T and Draksharishta-M can be a promising source of natural antioxidant.
Cite this article:
Preeti Tiwari, Rakesh K.Patel. Total Phenolics and Flavonoids and Antioxidant Potential of Draksharishta Prepared by Traditional and Modern Methods. Research J. Pharmacognosy and Phytochemistry 2012; 4(5): 244-249.