Author(s): C. S. Kandasamy, Sai Lohit Ch., R. Siva Kumar, V. Gopal, Chandini, R. Nair, R. Venkatanarayanan

Email(s): cskjss_98@yahoo.co.in

DOI: Not Available

Address: C. S. Kandasamy1*, Sai Lohit Ch.1, R. Siva Kumar1, V. Gopal2, Chandini, R. Nair3 and R. Venkatanarayanan1.
1Department of Pharmacognosy, R.V.S College of Pharmaceutical Sciences, Sulur, Coimbatore.
2Department of Pharmacognosy, College of Pharmacy, Mother Theresa Institute of Health Sciences and Research Institute, Pondicherry.
3Department of Biochemistry, SASTRA University, Tanjore.
*Corresponding Author

Published In:   Volume - 3,      Issue - 6,     Year - 2011


ABSTRACT:
Extracts of crude drugs such as Ajowan, Mace, Cardamom, Cumin, Nutmeg and Clove used in the Polyherbal formulation - RVSPHF567 were taken in account and dissolved in their appropriate solvents. These extracts were analyzed individually by High Performance Thin Layer Chromatography (HPTLC) to detect the presence of their phytoconstituents. The instrument used for High Performance Thin Layer Chromatography (HPTLC) was CAMAG LINOMAT 5. These analytical investigations were highly useful for preparing Polyherbal formulation - RVSPHF567 which is highly effective as Hepatoprotective agent and can be used in-vivo as a potential therapeutic agent. The sample was prepared by solvent extraction. Then 2µl of test solution was loaded as 6mm band length on the 9 x 10 Silica gel 60F254 TLC plate. This was done by using Hamilton syringe. These plates were kept in respective mobile phase Chloroform: Methanol: Water (6.5: 2.5: 0.4) for spot development to 90mm. These plates were dried and kept in Photo-documentation chamber - CAMAG REPROSTAR 3. The images were captured at White light, UV 254nm and UV 366nm. The developed plates were sprayed with respective spray reagent and photo-documented in White light and UV 366nm mode. Then the same were scanned at 500nm. The Peak table, Peak display and Peak Densitogram show the terpenoids profile in all the crude drugs.


Cite this article:
C. S. Kandasamy, Sai Lohit Ch., R. Siva Kumar, V. Gopal, Chandini, R. Nair, R. Venkatanarayanan. Analysis of Crude Drugs Present In the Hepatoprotective Polyherbal Formulation by HPTLC Technique. Research J. Pharmacognosy and Phytochemistry 2011; 3(6): 261-265.


Recomonded Articles:

Author(s): Anshuman Singh, Bhupendra Vyas

DOI: 10.5958/0975-4385.2018.00052.3         Access: Open Access Read More

Author(s): Punita R. Maurya, Swati R. Dhande, Yadunath M. Joshi, Vilasrao J. Kadam

DOI:         Access: Open Access Read More

Author(s): Bapu R. Thorat

DOI: 10.5958/0975-4385.2018.00017.1         Access: Open Access Read More

Author(s): Gomathi B , Anuradha R

DOI:         Access: Open Access Read More

Author(s): Vidula Salvi, Yadunath Joshi, Swati Dhande , Vilasrao Kadam

DOI:         Access: Open Access Read More

Author(s): Shantha Sheela Nagarajan, Muthusamy Periyannan , Radha Ramalingam

DOI: 10.5958/0975-4385.2016.00013.3         Access: Open Access Read More

Author(s): Shruti Srivastava, Jatin Jaiswal, Hemendra Gautam, Sunil Singh, Surabhi Sharma

DOI:         Access: Open Access Read More

Author(s): Lenkalapally Matsyagiri, Kommu Sudhakar, Takkadpelliwar Santoshi, Masna Nagarjuna , N. L. Gowrishankar

DOI:         Access: Open Access Read More

Author(s): Soosamma John, Madhavi T., Bincy Raj, Jincy Shaji, Vinutha

DOI:         Access: Open Access Read More

Author(s): Shantha Sheela Nagarajan, Muthusamy Periyanna, Radha Ramalingam

DOI: 10.5958/0975-4385.2015.00036.9         Access: Open Access Read More

Author(s): Okoro Ebere Kelechi, Amaku Friday James, Okoro Innocent Ajah

DOI: 10.5958/0975-4385.2016.00028.5         Access: Open Access Read More

Author(s): Umesh Annappan, Vijaya Bharathi Rajkishore, Radha Ramalingam

DOI: 10.5958/0975-4385.2015.00034.5         Access: Open Access Read More

Author(s): Ravindra Singh, Aakanksha Tiwari

DOI: 10.5958/0975-4385.2017.00009.7         Access: Open Access Read More

Author(s): Jadhav Santosh, Sawale Jyotiram

DOI: 10.5958/0975-4385.2016.00016.9         Access: Open Access Read More

Author(s): Kalpana Patil, Swati Dhande, Vilasrao Kadam

DOI:         Access: Open Access Read More

Author(s): Mamta Tiwari, Pushpraj S. Gupta, Nisha Sharma

DOI: 10.5958/0975-4385.2018.00014.6         Access: Open Access Read More

Author(s): Dhanya Rajan, J. Suresh, N. Paramakrishnan, A. Sri Vasavi Reddy, M Nayeem

DOI:         Access: Open Access Read More

Research Journal of Pharmacognosy and Phytochemistry (RJPP) is an international, peer-reviewed journal, devoted to pharmacognosy...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4385 


Recent Articles




Tags