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Volume No. :   3

Issue No. :  2

Year :  2011

ISSN Print :  0975-2331

ISSN Online :  0975-4385


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In-Vitro Antimicrobial Synergistic and Anti-Tb Activities of Opuntia dillenii Hydro-Alcoholic Extract



Address:   Santhosh Kumar C1*, Chiranjib Bhattacharjee1, Subal Debnath1, G. Ganesh Kumar1 and Atul N. Chandu2
1Srikrupa Institute of Pharmaceutical Sciences, Vil. Velkatta, Kondapak (MDL), Dist. Medak, Siddipet, Andhra Pradesh – 502 277. 2University Institute of Pharmacy, Pt. Ravishankar Sukla University, Raipur, C.G., India.
*Corresponding Author
DOI No:

ABSTRACT:
The objective of the present study was to evaluate the synergistic antimicrobial and anti-tubercular activities of Opuntia dillenii aqueous methanolic extract. Antibacterial study was carried out by plate hole diffusion or agar well diffusion assay to determine the growth inhibition of bacteria. Antifungal activity was performed by the use of Saubouraud dextrose agar medium (SDA). The synergistic activity study was calculated by means of Cup plate method (Kirbauy bauer technique) using two wells in a plate. The aqueous methanolic plant extract of Opuntia dillenii (500 µg/ml) was used in combination with oxytetracycline (500 µg/ml). Anti-tubercular assay was performed using Micro plate Alamar Blue Assay (MABA) using the suspension of Mycobacterium tuberculosis H37Rv strain. The concentrations of plant extract were used are 1000 µg/ml, 500 µg/ml, 250 µg/ml, 125 µg/ml, 62.5 µg/ml. The medicinal plant appear to have a broad antimicrobial activity spectrum, they could be useful in antiseptic and disinfectant formulation as well as in anti-tubercular activity. Among the various micro organisms, the aqueous methanolic extract was more active against Micrococcus leuteum, Proteus mirabilis. In antifungal activity of the aqueous methanolic extract shows positive results for all fungus. The anti-tubercular activities were compared with standard drug Rifampicin. The aqueous methanolic extract was having more percentage inhibition when compared to other extracts.
KEYWORDS:
Opuntia dillenii, Mycobacterium tuberculosis, Micro Plate Alamar Blue Assay.
Cite:
Santhosh Kumar C, Chiranjib Bhattacharjee, Subal Debnath, G. Ganesh Kumar, Atul N. Chandu. In-Vitro Antimicrobial Synergistic and Anti-Tb Activities of Opuntia dillenii Hydro-Alcoholic Extract. Research J. Pharmacognosy and Phytochemistry 2011; 3(2): 67-71.
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