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Research Journal of Pharmacognosy and Phytochemistry
ISSN: 0975-4385(Online), 0975-2331(Print)
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Optimization of process parameters for the production of L-asparaginase from an isolated fungus
P Mallikarjuna Rao
V Sreenivasulu1*, KN Jayaveera2 and P Mallikarjuna Rao3
1Department of Biotechnology, KLR Pharmacy College, Paloncha- 507115, Khammam (District), A.P, India.
2 Oil Technological Research Institute, JNT University, Anantapur–15001, A.P, India.
3 International Medical University, Kualalumpur, Malaysia-57000.
The extracellular L-asparaginase production by fungi isolated from soil samples by using pH and dye based method. Various physical and chemical parameters were optimized under submerged fermentation for L-asparaginase production. Maximum productivity of L-asparaginase (19.5 U/ml) was achieved by employing medium containing 2% (w/v) L-asparagine as substrate concentration, 1%(w/v) glucose as carbon source, 1% (w/v) ammonium sulphate as an additional nitrogen source with the incubation period of 96 h and incubation temperature at 30oC, initial pH 6.5 at an inoculum level 20% (v/v) with 48 h old inoculum was found to be optimum for maximum yield.
L-asparaginase, optimization and submerged fermentation
V Sreenivasulu, KN Jayaveera, P Mallikarjuna Rao. Optimization of process parameters for the production of L-asparaginase from an isolated fungus. Research J. Pharmacognosy and Phytochemistry 2009; 1(1):30-34.
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